Page No. 1 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B ________________________________________________________________________ Technology Trade Name: ANAEROBIC BIOTRANSFORMATION Address: 1220 Avenida Acaso City: Camarillo, California 93012 USA Contact: Greg Smith Title: Principal, Senior Program Manager Phone: (805) 388-3775 Fax: (805) 388-3377 Status: Full scale ________________________________________________________________________ DESCRIPTION OF TECHNOLOGY ENSR has designed and implemented a full-scale system combining several remediation technologies to treat soil and groundwater contaminated with chlorinated solvents. The system combines bioremediation, steam injection, soil vapor extraction, groundwater extraction, and air-stripping. This system has been effective in removing chlorinated solvents such as trichloroethane (TCA), and trichloroethene (TCE), present as dense nonaqueous phase liquids (DNAPLS). DNAPLs are present at the site in unconsolidated glacial clays with measured hydraulic permeabilities ranging from 10E-4 to 10E-5 cm/sec. The remediation approach for this technology combines the use of steam injection for the removal of dense nonaqueous phase liquids (DNAPL), coupled with the application of nutrients to groundwater to promote biotransformation of TCE and 1,1,1-TCA to compounds which are more easily removed from the subsurface. A network of groundwater injection wells is used to deliver deoxygenated steam to mobilize the DNAPL in the subsurface and maintain anaerobic conditions. The deoxygenated steam is applied in the DNAPL phase, and not in the aqueous phase. Injection wells are also used to deliver growth substrates and nutrients into the aqueous phase to promote the growth of indigenously occuring anaerobic bacteria. Anaerobic bacteria, specifically methanogenic bacteria, biotransform TCE and TCA to dichlorinated and monochlorinated alkenes and alkanes which are more easily removed from the subsurface in the aqueous phase. Although the kinetics associated with methanogenic bacteria are slow, the transformation is intended to only convert TCE and TCA to di-or mono-chlorinated compounds, making the remediated time faster. DNAPLs which are volatilized by close contact with injected steam are removed in the vapor phase by a network of vapor extraction wells. The vapor wells are connected to a vapor extraction unit which was custom built and designed for ENSR to be resistent to chemical corrosion. Air emissions from the vapor extraction unit are monitored using a total hydrocarbon analyzer. DNAPLs in the liquid phase are removed by a network of groundwater extraction wells. Extracted groundwater is treated in an air-stripper to remove chlorinated solvents. Page No. 2 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B TECHNOLOGY HIGHLIGHTS ENSR's in situ groundwater remediation process, based on a systems approach, its effective in biotransforming and removing high concentrations of chlorinated solvents, even if present as DNAPLs. The biotransformation is carried out under anaerobic conditions and delivery of oxygen to subsurface microrganisms is not required. This in situ application is well suited for remediation of areas located beneath building foundations. The conventional treatment approach for aquifers contaminated with these compounds is removal by groundwater extraction combined with aboveground treatment. ENSR's approach is to biotransform TCE and TCA under anaerobic conditions, producing dichlorinated and monochlorinated compounds. These compounds have different chemical characteristics than their parent compounds (such as move favorable aqueous solubilities, vapor pressures, and octanol-water and octanol-carbon partition coefficients), and hence are more easily removed from the subsurface. ENSR has estimated that approximately 30 groundwater pore volume flushes are required to remove 90% of the TCE from an aquifer consisting of glacial clays. Comparable removal of the degradation compounds of trans- and cis-1,2-dichloroethene only requires 4 to 6 pore volume flushes, respectively. Anaerobic biotransformation produces degradation compounds which require less volumes of groundwater to be removed, shortens the remediation timeframe, and therefore, reduces the cost of remediation. An innovative feature of this technology is the simultaneous use of steam injection and biostimulation. Biostimulation of anaerobic bacteria, specifically methanogenic bacteria, is done by addition of growth substrates (potassium acetate and sodium formate) and nutrients to the subsurface. The conversion of TCE and TCA into their degradation products is accomplished by co-metabolism process, where these chlorinated compounds are not utilized as a primary carbon source. Additions of growth substrates, such as potassium acetate and sodium formate may form organic acids. However, the acidity induced by these compounds can be neutralized by the buffering capacity of the groundwater. No pH decrease was observed in the past. Page No. 3 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B TECHNOLOGY LIMITATIONS This type of in situ remediation process is limited to the treatment of chlorinated solvents. Anaerobic bioremediation of most other organic compounds is less effective than aerobic biodegradation. Additionally, this technology does not treat metals. Implementation of this technology requires bench- or pilot-scale testing to aid in design of a full-scale system. The presence of iron in the treatment zone may result in clogging of groundwater extraction wells and air stripper tower packing material. However, more prevalent problem is scaling occurred due to excessive Ca+2 and Mg+2 ions concentrations. Injection of substrate and nutrients into groundwater will likely require a permit in most states. Care must be taken not to mobilize DNAPLs when applying this technology. Page No. 4 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B OTHER COMMENTS ON TECHNOLOGY ENSR has been operating a full scale remediation system based on biotransformation combined with steam injection for approximately two years. A design for remediation of a second site impacted by chlorinated solvents present as DNAPLs using a similar approach was completed in mid-1993. System installation and start-up is scheduled for 1994. Both sites where this technology is being applied are being remediated under privately funded voluntary cleanups. Cleanup objectives for these sites were negotiated with the appropriate state agency. Steam injection combined with biotransformation is likely to be an effective remedial technology for many sites impacted by releases of chlorinated solvents. ENSR has also designed, installed, and operated a number of more traditional in situ groundwater biotreatment systems for the remediation of BTEX contaminated aquifers. Page No. 5 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B WASTE APPLICATIONS Media Actual/Potential _ _ Soil (in situ) _ _ Soil (ex situ) _ _ Sludge (Does not include municipal sewage sludge) _ _ Solid (e.g., slag) _ X_ Natural sediment (in situ) _ _ Natural sediment (ex situ) X_ X_ Ground water (in situ) _ _ Off-gas generated from a primary innovative technology _ X_ Dense nonaqueous phase liquids (DNAPL) in situ _ X_ Light nonaqueous phase liquids (LNAPL) in situ Page No. 6 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B WASTE APPLICATIONS (Continued) Contaminants and Contaminant Groups Treated Actual/Potential Actual/Potential X_ X_ Halogenated volatiles _ _ Heavy metals _ _ Halogenated semivolatiles _ _ Nonmetallic toxic elements _ X_ Nonhalogenated volatiles _ _ Radioactive metals _ _ Nonhalogenated semivolatiles _ _ Asbestos _ _ Organic pesticides/herbicides _ _ Inorganic cyanides _ _ Dioxins/furans _ _ Inorganic corrosives _ _ PCBs _ _ Polynuclear aromatics (PNAs) Miscellaneous_____________ X_ X_ Solvents _ _ Explosives/propellents _ _ Benzene-toluene-ethylbenzene- _ _ Organometallic pesticides/ xylene (BTEX) herbicides _ _ Acetonitrile (organic cyanide) _ _ Organic acids Others: Page No. 7 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B WASTE APPLICATIONS (Continued) Industrial Waste Sources or Site Types Treated Actual/Potential Actual/Potential _ _ Agriculture _ X_ Munitions manufacturing _ _ Battery recycling/disposal _ X_ Paint/ink formulation _ _ Chloro-alkali manufacturing _ _ Pesticide manufacturing/use _ _ Coal gasification _ _ Petroleum refining and reuse _ X_ Dry cleaners _ X_ Photographic products _ X_ Electroplating _ _ Plastics manufacturing _ _ Gasoline/service station _ _ Pulp and paper industry _ _ Herbicide manufacturing/use _ X_ Other organic chemical manufacturing _ _ Industrial landfills _ _ Other inorganic chemical _ X_ Inorganic/organic pigments manufacturing _ _ Machine shops _ _ Rubber manufacturing _ _ Metal ore mining and smelting X_ X_ Semiconductor manufacturing _ X_ Municipal landfill _ _ Wood preserving Others: _ _ Uranium mining ---------- Page No. 8 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: AT&T ________________________________________ City: Confidential _________________________ State/Province: Illinois ________________ Country: USA _______________ Project Took Place at Site Named: Yes At another Site (i. e., a Test Facility): No Industrial Waste Sources or Site Types Treated _ Agriculture _ Paint/ink formulation _ Battery recycling/disposal _ Pesticide manufacturing/use _ Chloro-alkali manufacturing _ Petroleum refining and reuse _ Coal gasification _ Photographic products _ Dry cleaning _ Plastics manufacturing _ Electroplating _ Pulp and paper industry _ Gasoline service station _ Other organic chemical manuf. _ Herbicide manufacturing/use _ Other inorganic chemical manufacturing _ Industrial landfills _ Semiconductor manufacturing _ Inorganic/organics pigments _ Rubber manufacturing _ Machine shops _ Wood preserving _ Metal ore mining and smelting _ Uranium mining _ Municipal landfills _ Munitions manufacturing Others: __________ Page No. 9 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: AT&T ________________________________________ Regulation/Statute/Organization Media _ RCRA Corrective Action X_ Soil (in situ) _ CERCLA _ Soil (ex situ) _ TSCA _ Sludge _ Safe Drinking Water Act _ Solid _ UST Corrective Action _ Natural Sediment (in situ) _ DOD _ Natural Sediment (ex situ) _ DOE X_ Ground water (in situ) X_ State: Illinois _ Off-gas generated from a primary treatment technology _ Other: _ Dense nonaqueous phase liquid (in situ) _ Not Applicable _ Light nonaqueous phase liquid (in situ) Volume/Quantity Treated: __________ ____________(units) Area treated (for in situ projects): __________ ____________(units) Depth treated (for in sity projects): __________ ____________(units) Page No. 10 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: AT&T ________________________________________ Application Type X_ Full-scale cleanup _ TSCA Research and Development _ Field Demonstration _ EPA SITE Demonstration Program _ Pilot scale treatability study _ EPA SITE Emerging Technology Program _ RCRA Research, Development and Demo _ Research _ TSCA National Demonstration Other: __________ Estimated or actual total and/or unit cost for this project Cost: ____________ Units: _________________ Total: ____________ Page No. 11 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: AT&T ________________________________________ Performance Data Contaminant Untreated Concent. Treated Concent. ----------------------------- --------------------- --------------------- Trichloroethylene (TCE) 0.005 -86 mg/L 0.0002 -0.17 mg/L 1,1,1-Trichloroethane (TCA) 0.0005 -330 mg/L 0.0005 -0.936 mg/L TPH (Fuel Oil) 1,000 -20,000 mg/kg 250 -5,000 mg/kg BTEX 5,400 -20,000 mg/kg 100 -500 mg/kg PAHs 0 -17 mg/kg 0 -1 mg/kg Page No. 12 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: AT&T ________________________________________ Person outside company familiar with project (optional): Name: Angelo Basile ________________________________________ Company: AT&T ________________________________________ Address: 131 Morristown Road ________________________________________ Basking Ridge, NY 07920 ________________________________________ Additional Project Information: _______________ As of April 1993, approximately 13,285 pounds of hydrocarbons had been removed from the subsurface as measured by emissions from the air stripper and the vapor extraction system operating at one site. This quantity includes TCE as well as other chlorinated solvents. Page No. 13 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B TECHNICAL REFERENCES Author(s): G.J. Smith and G.A. Ferguson, ENSR ______________________________________________________________________ ______________________________________________________________________ Title: In-Situ Remediation Using Anaerobic Biotransformation ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: 2nd International In Situ and On-Site Bioreclamation ______________________________________________________________________ ______________________________________________________________________ Date: 04/93 ________ NTIS/EPA Document Number(s): _________________________ Page No. 14 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B TECHNICAL REFERENCES Author(s): Smith, G.J., J.W. Aiken, and J.F. Tursman, ENSR ______________________________________________________________________ ______________________________________________________________________ Title: A Systems Approach for Rapid Cleanup of Chlorinated Solvent ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: Presented at 1 USA/USSR Joint Conference on Enviornmental Hydrology ______________________________________________________________________ ______________________________________________________________________ Date: 06/90 ________ NTIS/EPA Document Number(s): _________________________ Page No. 15 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B TECHNICAL REFERENCES Author(s): Smith, G. J. an J.W. Aiken, ENSR ______________________________________________________________________ ______________________________________________________________________ Title: In-Situ Remediation of Chlorinated Solvents in Soils and GW ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: 84th Annual Air and Waste Management Association Meeting and Exhibit ______________________________________________________________________ ______________________________________________________________________ Date: 06/91 ________ NTIS/EPA Document Number(s): _________________________ Page No. 16 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B TECHNICAL REFERENCES Author(s): Chacanos, J.D., R.W. Hunter and S.S. Huismann, ENSR ______________________________________________________________________ ______________________________________________________________________ Title: A Lesson in Site Management and Cleanup: The Bishop Tank Farm Rn ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: Society of Petroleum Engineering, Western Regionall Meeting, Anch ______________________________________________________________________ ______________________________________________________________________ Date: 01/93 ________ NTIS/EPA Document Number(s): _________________________ Page No. 17 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B ESTIMATED PRICE RANGE Estimated price range per unit of waste treated : $ 120 __________ to $ 160 __________ per pound solvent _______________ Price estimates shown above do not always include all indirect costs associated with treatment, such as excavation, permits and treatment of residuals. For price comparisons, users should make certain that vendors provide estimates based on comparable remediation activities. Factors that have a significant effect on unit price. (1 is highest). 4__ Initial contaminant concentration __ Moisture content of soil 5__ Target contaminant concentration 6__ Site Preparation 1__ Quantity of waste __ Waste handling/preprocessing 2__ Depth of contamination __ Amount of debris with waste 3__ Depth to ground water __ Characteristics of soil 9__ Characteristics of residual waste 8__ Utility/Fuel rates 7__ Labor Rates Others: __________ Page No. 18 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B FULL SCALE INFORMATION Major unit processes : Preprocess - Install steam injection wells - Install nutrient injection wells - Install network of vapor extraction and groundwater extraction wells - Size extraction and injection pumps and air stripping tower. 1. Monitoring and injection/withdrawal wells are drilled according to design specifications. 2. Steam generation and pumping equipment is installed. 3. Any pretreatment or post treatment process units are installed based on treatability testing. Typically, settling tanks and iron removal equipment are required as pretreatment for the air stripping unit. Process - Injection of deoxygenated steam to mobilize or volatilize DNAPLs. - Nutrient and substrate injection to enhance biotranformation. - Vapor extraction to remove aqueous phase solvents. - Removal of suspended solids in groundwater via a settling tank. - Air stripper process for removal chlorinated solvents from groundwater. 1. A network of thermistor probes is used to measure changes in temperature at varying depths and distance from steam injection wells. 2. Monitoring wels and extraction wells are monitored for nutrients, chloride, total organic halides, and target compounds. 3. System controls and safety devices are monitored 24 hours a day by a microcomputer with remote data transfer capabilities. Page No. 19 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: ENSR CONSULTING AND ENGINEERING Technology Type: BIOREMEDIATION - IN SITU GROUND WATER (s0B FULL SCALE INFORMATION (Continued) Vendor services: _ Equipment manufacture _ Subcontractor for cleanup services X_ Prime contractor for full-service remediation _ Other: Full-scale facility is: _ Transportable _ Fixed X_ In situ Number of full-scale cleanups initiated or completed by this firm using this technology: 1______ Number of full-scale systems: 2_____ Planned/in design 0_____ Under construction 1_____ Constructed Capacity range: NA ____________ to ____________ _______________ For equipment manufacturers - estimated or actual number of full-scale cleanups by other firms using this equipment: 0______ Page No. 1 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B ________________________________________________________________________ Technology Trade Name: MIXFLO (TM) Address: Linde Division - M33 777 Old Saw Mill River Road City: Tarrytown, New York 10591 USA Contact: Gary E. Storms Title: Applications Manager Phone: (914) 789-3034 Fax: (914) 789-2341 Status: Full scale ________________________________________________________________________ DESCRIPTION OF TECHNOLOGY Praxair's MIXFLO System dissolves oxygen into a slurry from a lagoon in a two-stage process. In the first stage, slurry is pumped from the lagoon and pressurized to between 2 and 4 atmospheres. Pure oxygen gas is injected into the slurry. The resulting mixture passes through a pipeline contactor in which approximately 60 percent of the injected oxygen dissolves. In the second stage, the oxygen/slurry mixture is reinjected into the lagoon through a liquid/liquid eductor which ingests unoxygenated slurry from the lagoon and mixes it with the oxygenated slurry. This technology can also be used to assist a slurry-phase bioremediation system. Page No. 2 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B TECHNOLOGY HIGHLIGHTS Praxair's MIXFLO System is compatible with all aerobic lagoon phase bioremediation processes that would otherwise use air. Major benefits of the system are: 1. VOC emissions are reduced by over 99 percent compared to conventional aeration systems, resulting in reduced ambient air monitoring and emissions abatement costs. 2. Reduced power consumption 3. Improved community relations 4. Improved on-line factor 5. Potential increase in biodegradation rate 6. Reduced evaporative cooling 7. Reduced foaming Page No. 3 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B TECHNOLOGY LIMITATIONS 1. This technology is only applicable to slurry-phase type of bioremediation. Therefore, as an in situ process, it is applicable to lagoons, pits, ponds, etc. 2. Applicable only to aerobically biodegradable components (similar to air-based processes). 3. Slurry must be pumpable and pass through a 1/2-inch mesh. Page No. 4 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B OTHER COMMENTS ON TECHNOLOGY 1. Over 150 secondary wastewater treatment installations, including both tanks and lagoons, are successfully using this technology in Europe and South America. 2. A full-scale in situ lagoon phase bioremediation began in early 1992 at the French, Ltd. Superfund site in Crosby, Texas using the MIXFLO technology. The technology met all its design expectations and the remediation was completed ahead of schedule. Page No. 5 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B WASTE APPLICATIONS Media Actual/Potential X_ X_ Soil (in situ) _ _ Soil (ex situ) _ _ Sludge (Does not include municipal sewage sludge) _ X_ Solid (e.g., slag) X_ X_ Natural sediment (in situ) _ _ Natural sediment (ex situ) _ _ Ground water (in situ) _ _ Off-gas generated from a primary innovative technology _ _ Dense nonaqueous phase liquids (DNAPL) in situ _ _ Light nonaqueous phase liquids (LNAPL) in situ Page No. 6 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B WASTE APPLICATIONS (Continued) Contaminants and Contaminant Groups Treated Actual/Potential Actual/Potential X_ X_ Halogenated volatiles _ _ Heavy metals _ X_ Halogenated semivolatiles _ _ Nonmetallic toxic elements X_ X_ Nonhalogenated volatiles _ _ Radioactive metals X_ X_ Nonhalogenated semivolatiles _ _ Asbestos _ X_ Organic pesticides/herbicides _ _ Inorganic cyanides _ _ Dioxins/furans _ _ Inorganic corrosives X_ X_ PCBs X_ X_ Polynuclear aromatics (PNAs) Miscellaneous_____________ X_ X_ Solvents _ _ Explosives/propellents X_ X_ Benzene-toluene-ethylbenzene- _ _ Organometallic pesticides/ xylene (BTEX) herbicides _ _ Acetonitrile (organic cyanide) _ _ Organic acids Others: Page No. 7 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B WASTE APPLICATIONS (Continued) Industrial Waste Sources or Site Types Treated Actual/Potential Actual/Potential _ X_ Agriculture _ _ Munitions manufacturing _ _ Battery recycling/disposal _ _ Paint/ink formulation _ _ Chloro-alkali manufacturing _ _ Pesticide manufacturing/use _ X_ Coal gasification X_ X_ Petroleum refining and reuse _ X_ Dry cleaners _ _ Photographic products _ _ Electroplating _ _ Plastics manufacturing _ _ Gasoline/service station _ X_ Pulp and paper industry _ _ Herbicide manufacturing/use X_ X_ Other organic chemical manufacturing _ X_ Industrial landfills _ _ Other inorganic chemical _ _ Inorganic/organic pigments manufacturing _ _ Machine shops _ _ Rubber manufacturing _ _ Metal ore mining and smelting _ _ Semiconductor manufacturing _ X_ Municipal landfill _ X_ Wood preserving Others: _ _ Uranium mining ---------- Page No. 8 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: French, Ltd. *________________________________________ City: Crosby _________________________ State/Province: Texas ________________ Country: USA _______________ Project Took Place at Site Named: Yes At another Site (i. e., a Test Facility): No Industrial Waste Sources or Site Types Treated _ Agriculture _ Paint/ink formulation _ Battery recycling/disposal _ Pesticide manufacturing/use _ Chloro-alkali manufacturing _ Petroleum refining and reuse _ Coal gasification _ Photographic products _ Dry cleaning _ Plastics manufacturing _ Electroplating _ Pulp and paper industry _ Gasoline service station _ Other organic chemical manuf. _ Herbicide manufacturing/use _ Other inorganic chemical manufacturing _ Industrial landfills _ Semiconductor manufacturing _ Inorganic/organics pigments _ Rubber manufacturing _ Machine shops _ Wood preserving _ Metal ore mining and smelting _ Uranium mining _ Municipal landfills _ Munitions manufacturing Others: __________ mixed organic wastes Page No. 9 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: French, Ltd. *________________________________________ Regulation/Statute/Organization Media _ RCRA Corrective Action X_ Soil (in situ) X_ CERCLA _ Soil (ex situ) _ TSCA X_ Sludge _ Safe Drinking Water Act _ Solid _ UST Corrective Action X_ Natural Sediment (in situ) _ DOD _ Natural Sediment (ex situ) _ DOE _ Ground water (in situ) _ State: _ Off-gas generated from a primary treatment technology _ Other: _ Dense nonaqueous phase liquid (in situ) _ Not Applicable _ Light nonaqueous phase liquid (in situ) Volume/Quantity Treated: 140,000 __________ tons ____________(units) Area treated (for in situ projects): 7 __________ acres ____________(units) Depth treated (for in sity projects): 15 __________ feet ____________(units) Page No. 10 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: French, Ltd. *________________________________________ Application Type X_ Full-scale cleanup _ TSCA Research and Development _ Field Demonstration _ EPA SITE Demonstration Program _ Pilot scale treatability study _ EPA SITE Emerging Technology Program _ RCRA Research, Development and Demo _ Research _ TSCA National Demonstration Other: __________ Estimated or actual total and/or unit cost for this project Cost: ____________ Units: _________________ Total: ____________ Page No. 11 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: French, Ltd. *________________________________________ Performance Data Contaminant Untreated Concent. Treated Concent. ----------------------------- --------------------- --------------------- Page No. 12 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: French, Ltd. *________________________________________ Person outside company familiar with project (optional): Name: Dick Sloan, Project Manager ________________________________________ Company: FLTG, INCORPORATED ________________________________________ Address: 15010 FM 2100; Suite 200, Crosby, TX ________________________________________ 77532 (713) 328-1941 ________________________________________ Additional Project Information: _______________ Lagoon sludge and subsoil contaminated with industrial oils; acidic,galvanizing waste; phenols, PCBs, PNAs (140,000 cubic yards) Praxair's parnter in the remediation of the lagoon at French, Ltd. was ENSR. ENSR is also a VISITT vendor. Page No. 13 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B TECHNICAL REFERENCES Author(s): ENSR Consulting and Engineering ______________________________________________________________________ ______________________________________________________________________ Title: BIOREMEDIATION PROCESS DESIGN REPORT, FRENCH LIMITED ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: ______________________________________________________________________ ______________________________________________________________________ Date: 12/90 ________ NTIS/EPA Document Number(s): SITE Doc. No.2870-017-552_________________________ Page No. 14 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B TECHNICAL REFERENCES Author(s): ENSR Consulting and Engineering ______________________________________________________________________ ______________________________________________________________________ Title: A Comparison of Fine-Bubble Diffuser Aeration versus Linde ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: ______________________________________________________________________ ______________________________________________________________________ Date: 11/90 ________ NTIS/EPA Document Number(s): _________________________ Page No. 15 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B TECHNICAL REFERENCES Author(s): ENSR Consulting and Engineering ______________________________________________________________________ ______________________________________________________________________ Title: Design Report Executive Summary/Dick Sloan, ARCO ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: ______________________________________________________________________ ______________________________________________________________________ Date: 12/90 ________ NTIS/EPA Document Number(s): _________________________ Page No. 16 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B TECHNICAL REFERENCES Author(s): Bergman, T.J., Jr.; Greene, J.M.; Davis, T.R. ______________________________________________________________________ ______________________________________________________________________ Title: An In-Situ Slurry-Phase Bioremediation Case... ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: In Situ Treatment of Contaminated Soil & Water Symposium ______________________________________________________________________ ______________________________________________________________________ Date: 02/92 ________ NTIS/EPA Document Number(s): _________________________ Page No. 17 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B TECHNICAL REFERENCES Author(s): Praxair, Inc., Linde Division ______________________________________________________________________ ______________________________________________________________________ Title: Oxygen for Wastewater Treatment using the Mixflo System ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: ______________________________________________________________________ ______________________________________________________________________ Date: 01/92 ________ NTIS/EPA Document Number(s): _________________________ Page No. 18 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B TECHNICAL REFERENCES Author(s): Praxair, Inc., Linde Division ______________________________________________________________________ ______________________________________________________________________ Title: Gas-Liquid Mixing Technology for the Chemicals Industry ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: ______________________________________________________________________ ______________________________________________________________________ Date: 07/92 ________ NTIS/EPA Document Number(s): _________________________ Page No. 19 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B TECHNICAL REFERENCES Author(s): French Limited ______________________________________________________________________ ______________________________________________________________________ Title: Bioremediation Process Design Report ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: SITE Document No. 2870-017-552 ______________________________________________________________________ ______________________________________________________________________ Date: / ________ NTIS/EPA Document Number(s): SITE Doc. No.2870-017-552_________________________ Page No. 20 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B ESTIMATED PRICE RANGE Estimated price range per unit of waste treated : $ 100.00 __________ to $ 300.00 __________ per ton _______________ Price estimates shown above do not always include all indirect costs associated with treatment, such as excavation, permits and treatment of residuals. For price comparisons, users should make certain that vendors provide estimates based on comparable remediation activities. Factors that have a significant effect on unit price. (1 is highest). 1__ Initial contaminant concentration __ Moisture content of soil 3__ Target contaminant concentration __ Site Preparation 2__ Quantity of waste __ Waste handling/preprocessing 4__ Depth of contamination __ Amount of debris with waste __ Depth to ground water __ Characteristics of soil __ Characteristics of residual waste __ Utility/Fuel rates __ Labor Rates Others: __________ Page No. 21 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B FULL SCALE INFORMATION Major unit processes : Slurry is pumped from the lagoon and pressured to between 2 and 4 atmospheres. Pure oxygen is then finely dispersed into the slurry. The resulting two-phase mixture passes through a pipeline contactor in which 60 percent of the injected oxygen dissolves due to elevated pressure and increased oxygen solubility. The oxygen/slurry dispersion is reinjected into the lagoon using a liquid/liquid eductor which ingests unoxygenated slurry, mixes it with the oxygenated slurry, and then disperses the mixture in the lagoon. The remaining oxygen bubbles are well dispersed in the lagoon and dissolve readily. Overall oxygen utilization efficiency is over 90 percent in the lagoon. The MIXFLO system is used to replace conventional aeration systems. Praxair does not offer the over all bioremediation design or operation. Page No. 22 of 22 VISITT 3.0 11/30/99 (s3B Vendor Name: PRAXAIR, INC. (FORMERLY UNION CARBIDE) Technology Type: BIOREMEDIATION - IN SITU LAGOON (s0B FULL SCALE INFORMATION (Continued) Vendor services: _ Equipment manufacture X_ Subcontractor for cleanup services _ Prime contractor for full-service remediation X_ Other: Industrial gas supplier Full-scale facility is: _ Transportable _ Fixed X_ In situ Number of full-scale cleanups initiated or completed by this firm using this technology: 150______ Number of full-scale systems: 0_____ Planned/in design 0_____ Under construction 1_____ Constructed Capacity range: ____________ to ____________ _______________ For equipment manufacturers - estimated or actual number of full-scale cleanups by other firms using this equipment: 0______ Page No. 1 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B ________________________________________________________________________ Technology Trade Name: FYREZYME Address: 6119 E. Star Valley Street City: Mesa, Arizona 85205 USA Contact: Mr. Pete Condy Title: CEO Phone: (602) 985-5524 Fax: (602) 985-2988 Status: Full scale ________________________________________________________________________ DESCRIPTION OF TECHNOLOGY FyreZyme is a multifactoral liquid (aqueous) agent, combining a rich source of bacterial growth-enhancing agents, extracellular enzymes, and bioemulsifiers. The bacterial growth enhancers induce a logarithmic growth phase in native soil bacteria, while the extracellular enzymes initiate the oxidative process in petroleum contaminant oxidation. The enhanced bacterial population adapts to the presence of partially-oxidized contaminant, utilizing it as a source for further preferential growth of petroleum-degrading organisms, both aerobic and anaerobic. Biodegradable bioemulsifiers/ surfactants help move the aqueous solution through the soil, mobilizing petroleum and cleaning blocked soil pore spaces. For in situ treatment, a "front" of FyreZyme-containing aerated water is moved through the soil by a combination of infiltration galleries or injection wells upgradient and extraction well(s) downgradient. It is necessary to maintain aerobic conditions through use of peroxide, injection wells, bioventing, sparging, etc. for maximal rates of bioremediation. This technology can be added to and increase the effectiveness of other technologies such as bioventing, air sparging, soil vacuum extraction, etc. Can also be used to create a bioremediation undergound "fence" to contain plumes. FyreZyme is non-toxic to the standard EPA-recommended test of marine vertebrate and invertebrate forms, and non-toxic by ingestion or chronic inhalation in Spargue-Dawley rats, and guinea pigs. It produces negative sensitivity in skin and conjunctival application to New Zealand white rabbits. It is authorized for use by On-Scene Commander on all navigable waterways in the US by EPA, with standard disclaimer of endorsement or approval by that agency. (This technology is also listed under Bioremediation - in situ groundwater. For off-gas treatment, see the description for off-gas treatment.) Page No. 2 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNOLOGY HIGHLIGHTS FyreZyme is a concentrated aqueous solution, which is diluted to 4,5, or 6 percent for use as a bioremediation enhancing agent. FyreZyme is stable, with a shelf life of 5 years under "normal" environmental conditions. FyreZyme is effective in accelerating bioremediation of a wide range of organic contaminants in an environmentally friendly, scientifically sound, and cost-effective manner. FyreZyme may be used as a stand-alone technology or incorporated into other technologies such as bioventing, soil venting, sparging, slurry phase treatment, sediment treatment, pump-and-treat, etc.. Among the advantages to FyreZyme are its ease of use, permanent solution to contamination, and affordability. When used to eliminate volatile organic compounds (VOC), the cost is less than 10 percent that of granulated activated carbon (GAC). When used as primary bioremediation agent, the cost ranges from $6-8 per cubic yard. If added to ongoing technologies mentioned above, the cost is negligible, compared with expenditures on typical phase-transfer technologies. Page No. 3 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNOLOGY LIMITATIONS The main limitations are those that limit bacterial growth, such as temperature extremes, pH below 3 or above 10, and bactericidal contaminants in high concentrations. Enzyme poisons must be flooded with FyreZyme before bioremediation will ensue. Aerobic conditions must be attained, at least periodically, to ensure complete metabolism of contaminants to non-toxic end products. In soils with low permeability, movement of aerated water and FyreZyme through soil is noticeably faster than that of ground water, due to presence of biodegradable emulisifiers/surfactants. If temperature is above 100 degrees Fahrenheit, the shelf life of FyreZyme may be shortened to 2 years from 5 years. Page No. 4 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B OTHER COMMENTS ON TECHNOLOGY An in situ field program is in progress in California and Nevada, using a combination of upgradient vertical and horizontal injection wells and a downgradient extraction well. A similar program is being engineered at a Waco, Texas site with Technologies International, Inc. Page No. 5 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B WASTE APPLICATIONS Media Actual/Potential X_ X_ Soil (in situ) _ _ Soil (ex situ) _ X_ Sludge (Does not include municipal sewage sludge) _ X_ Solid (e.g., slag) _ X_ Natural sediment (in situ) _ _ Natural sediment (ex situ) _ X_ Ground water (in situ) _ _ Off-gas generated from a primary innovative technology _ X_ Dense nonaqueous phase liquids (DNAPL) in situ _ X_ Light nonaqueous phase liquids (LNAPL) in situ Page No. 6 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B WASTE APPLICATIONS (Continued) Contaminants and Contaminant Groups Treated Actual/Potential Actual/Potential X_ X_ Halogenated volatiles _ _ Heavy metals X_ X_ Halogenated semivolatiles _ X_ Nonmetallic toxic elements X_ X_ Nonhalogenated volatiles _ _ Radioactive metals X_ X_ Nonhalogenated semivolatiles _ _ Asbestos _ X_ Organic pesticides/herbicides _ _ Inorganic cyanides _ X_ Dioxins/furans _ X_ Inorganic corrosives _ X_ PCBs X_ X_ Polynuclear aromatics (PNAs) Miscellaneous_____________ X_ X_ Solvents _ X_ Explosives/propellents X_ X_ Benzene-toluene-ethylbenzene- _ X_ Organometallic pesticides/ xylene (BTEX) herbicides _ _ Acetonitrile (organic cyanide) _ X_ Organic acids Others: Precipitates heavy metals from effluent with high BOD, reducing cost Page No. 7 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B WASTE APPLICATIONS (Continued) Industrial Waste Sources or Site Types Treated Actual/Potential Actual/Potential X_ X_ Agriculture _ X_ Munitions manufacturing _ _ Battery recycling/disposal _ X_ Paint/ink formulation _ X_ Chloro-alkali manufacturing _ X_ Pesticide manufacturing/use _ X_ Coal gasification X_ X_ Petroleum refining and reuse _ X_ Dry cleaners _ X_ Photographic products _ X_ Electroplating X_ X_ Plastics manufacturing X_ X_ Gasoline/service station _ X_ Pulp and paper industry _ X_ Herbicide manufacturing/use X_ X_ Other organic chemical manufacturing X_ X_ Industrial landfills _ _ Other inorganic chemical _ _ Inorganic/organic pigments manufacturing X_ X_ Machine shops X_ X_ Rubber manufacturing _ _ Metal ore mining and smelting _ X_ Semiconductor manufacturing _ X_ Municipal landfill X_ X_ Wood preserving Others: _ _ Uranium mining ---------- USDA currently is working with FyreZyme to bioremediate pesticides utilized for cattle-dip operations along the Mexican border. It is included in USDA research activities for the upcoming year. Page No. 8 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Tahoe ________________________________________ City: _________________________ State/Province: California ________________ Country: USA _______________ Project Took Place at Site Named: Yes At another Site (i. e., a Test Facility): No Industrial Waste Sources or Site Types Treated _ Agriculture _ Paint/ink formulation _ Battery recycling/disposal _ Pesticide manufacturing/use _ Chloro-alkali manufacturing X_ Petroleum refining and reuse _ Coal gasification _ Photographic products _ Dry cleaning _ Plastics manufacturing _ Electroplating _ Pulp and paper industry X_ Gasoline service station _ Other organic chemical manuf. _ Herbicide manufacturing/use _ Other inorganic chemical manufacturing _ Industrial landfills _ Semiconductor manufacturing _ Inorganic/organics pigments _ Rubber manufacturing _ Machine shops _ Wood preserving _ Metal ore mining and smelting _ Uranium mining _ Municipal landfills _ Munitions manufacturing Others: __________ Page No. 9 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Tahoe ________________________________________ Regulation/Statute/Organization Media _ RCRA Corrective Action X_ Soil (in situ) _ CERCLA _ Soil (ex situ) _ TSCA _ Sludge _ Safe Drinking Water Act _ Solid _ UST Corrective Action _ Natural Sediment (in situ) _ DOD _ Natural Sediment (ex situ) _ DOE _ Ground water (in situ) X_ State: Nevada, Califor _ Off-gas generated from a primary treatment technology _ Other: _ Dense nonaqueous phase liquid (in situ) _ Not Applicable _ Light nonaqueous phase liquid (in situ) Volume/Quantity Treated: 3,000 __________ cubic yards ____________(units) Area treated (for in situ projects): __________ ____________(units) Depth treated (for in sity projects): >150 __________ feet ____________(units) Page No. 10 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Tahoe ________________________________________ Application Type X_ Full-scale cleanup _ TSCA Research and Development _ Field Demonstration _ EPA SITE Demonstration Program _ Pilot scale treatability study _ EPA SITE Emerging Technology Program _ RCRA Research, Development and Demo _ Research _ TSCA National Demonstration Other: __________ Estimated or actual total and/or unit cost for this project Cost: 6 ____________ Units: cubic yard _________________ Total: ____________ Page No. 11 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Tahoe ________________________________________ Performance Data Contaminant Untreated Concent. Treated Concent. ----------------------------- --------------------- --------------------- TPH 1,200 -2,000 mg/kg Page No. 12 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Tahoe ________________________________________ Person outside company familiar with project (optional): Name: Project Manager ________________________________________ Company: Western Technologies ________________________________________ Address: Tempe, Arizona ________________________________________ ________________________________________ Additional Project Information: _______________ Mass balance studies have been conducted utilizing EPA-standard soil, fuel oil #2, and Kuwaiti crude. No treated concentration ranges are available at this time. Page No. 13 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Confidential ________________________________________ City: Waco _________________________ State/Province: Texas ________________ Country: USA _______________ Project Took Place at Site Named: Yes At another Site (i. e., a Test Facility): No Industrial Waste Sources or Site Types Treated _ Agriculture _ Paint/ink formulation _ Battery recycling/disposal _ Pesticide manufacturing/use _ Chloro-alkali manufacturing X_ Petroleum refining and reuse _ Coal gasification _ Photographic products _ Dry cleaning _ Plastics manufacturing _ Electroplating _ Pulp and paper industry X_ Gasoline service station X_ Other organic chemical manuf. _ Herbicide manufacturing/use _ Other inorganic chemical manufacturing _ Industrial landfills _ Semiconductor manufacturing _ Inorganic/organics pigments _ Rubber manufacturing X_ Machine shops _ Wood preserving _ Metal ore mining and smelting _ Uranium mining _ Municipal landfills _ Munitions manufacturing Others: __________ Tire manufacturing facility. Page No. 14 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Confidential ________________________________________ Regulation/Statute/Organization Media _ RCRA Corrective Action X_ Soil (in situ) _ CERCLA _ Soil (ex situ) _ TSCA _ Sludge _ Safe Drinking Water Act _ Solid _ UST Corrective Action _ Natural Sediment (in situ) _ DOD _ Natural Sediment (ex situ) _ DOE _ Ground water (in situ) X_ State: Texas _ Off-gas generated from a primary treatment technology _ Other: _ Dense nonaqueous phase liquid (in situ) _ Not Applicable _ Light nonaqueous phase liquid (in situ) Volume/Quantity Treated: 6,000 __________ cubic yards ____________(units) Area treated (for in situ projects): __________ ____________(units) Depth treated (for in sity projects): 30 __________ feet ____________(units) Page No. 15 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Confidential ________________________________________ Application Type X_ Full-scale cleanup _ TSCA Research and Development _ Field Demonstration _ EPA SITE Demonstration Program _ Pilot scale treatability study _ EPA SITE Emerging Technology Program _ RCRA Research, Development and Demo _ Research _ TSCA National Demonstration Other: __________ Estimated or actual total and/or unit cost for this project Cost: NA ____________ Units: _________________ Total: ____________ Page No. 16 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Confidential ________________________________________ Performance Data Contaminant Untreated Concent. Treated Concent. ----------------------------- --------------------- --------------------- TPH 840 -8,000 mg/kg Page No. 17 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Confidential ________________________________________ Person outside company familiar with project (optional): Name: Eric Fable ________________________________________ Company: Technologies International ________________________________________ Address: Scottsdale, AZ ________________________________________ ________________________________________ Additional Project Information: _______________ Construction of infiltration gallery, injection and extraction wells were completed in February, 1993. Project is underway, and no treated concentrations are available at this time. Page No. 18 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNICAL REFERENCES Author(s): Birge, John, and Wm.C. Brown ______________________________________________________________________ ______________________________________________________________________ Title: Modern Microbiology Principles & Applications ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: Dubuque, IA ______________________________________________________________________ ______________________________________________________________________ Date: 1992 ________ NTIS/EPA Document Number(s): _________________________ Page No. 19 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNICAL REFERENCES Author(s): Glaser, John A. ______________________________________________________________________ ______________________________________________________________________ Title: Enzyme Systems and Related Reactive Species ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: U.S. Enviornmental Protection Agency, Cincinnati, Ohio ______________________________________________________________________ ______________________________________________________________________ Date: 1992 ________ NTIS/EPA Document Number(s): _________________________ Page No. 20 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNICAL REFERENCES Author(s): Meaders, R.H., & J.O. Sipperley ______________________________________________________________________ ______________________________________________________________________ Title: FyreZyme Toxicity Studies ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: Global Marine Technologies, Mesa, Arizona ______________________________________________________________________ ______________________________________________________________________ Date: 1992 ________ NTIS/EPA Document Number(s): _________________________ Page No. 21 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNICAL REFERENCES Author(s): Priest, Fergus G. ______________________________________________________________________ ______________________________________________________________________ Title: Extracellur Enzymes ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: Van Nostrand Reinhold (U.K.) Co. Ltd. Workingham Bekshire ______________________________________________________________________ ______________________________________________________________________ Date: 1984 ________ NTIS/EPA Document Number(s): _________________________ Page No. 22 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNICAL REFERENCES Author(s): Shorthouse, Barry T. ______________________________________________________________________ ______________________________________________________________________ Title: The Process and Potential of Bioremediation Remediation ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: Remediation 1(1): 31-41 ______________________________________________________________________ ______________________________________________________________________ Date: 1990 ________ NTIS/EPA Document Number(s): _________________________ Page No. 23 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNICAL REFERENCES Author(s): Thibault, G.T. & N.W. Elliott ______________________________________________________________________ ______________________________________________________________________ Title: Accelerating the Biological Clean-up of Hazardous Spills ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: Prevention, Control, Cleanup, Info. Transfer, Etc., P115-120 ______________________________________________________________________ ______________________________________________________________________ Date: 1979 ________ NTIS/EPA Document Number(s): _________________________ Page No. 24 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNICAL REFERENCES Author(s): Paul, E.A. and F.E. Clark ______________________________________________________________________ ______________________________________________________________________ Title: Soil Microbiology and Biochemistry ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: Academica Press, Inc., San Diego, CA ______________________________________________________________________ ______________________________________________________________________ Date: 1988 ________ NTIS/EPA Document Number(s): _________________________ Page No. 25 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNICAL REFERENCES Author(s): Sims, Judith L., R.C. Sims, and J.E. Matthews. ______________________________________________________________________ ______________________________________________________________________ Title: Approach to Bioremediation of Contaminated Soils ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: Hazardous Waste & Hazardous Materials, 7(2): 117-149 ______________________________________________________________________ ______________________________________________________________________ Date: 1990 ________ NTIS/EPA Document Number(s): _________________________ Page No. 26 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNICAL REFERENCES Author(s): ______________________________________________________________________ ______________________________________________________________________ Title: Utilizing Bioremediation Technologies: Difficulties & Approaches ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: Interdisc. Bioremediation Working Group, Rutgers, NJ ______________________________________________________________________ ______________________________________________________________________ Date: 1991 ________ NTIS/EPA Document Number(s): _________________________ Page No. 27 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNICAL REFERENCES Author(s): Lee, M.D. and others ______________________________________________________________________ ______________________________________________________________________ Title: Biorestoration of Aquifiers Contaminated with Organic Compounds ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: CRC Critical Reviews in Environmental Control, 18(1) ______________________________________________________________________ ______________________________________________________________________ Date: 1988 ________ NTIS/EPA Document Number(s): _________________________ Page No. 28 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNICAL REFERENCES Author(s): Newman, Wm. and M.M. Marinson ______________________________________________________________________ ______________________________________________________________________ Title: Let Biodegradation Promote In Situ Venting ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: Remediation, 2(3): 277-293 ______________________________________________________________________ ______________________________________________________________________ Date: 1992 ________ NTIS/EPA Document Number(s): _________________________ Page No. 29 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNICAL REFERENCES Author(s): Semprini, L. D. Grbic-Galic, P. McCarty, and P. Roberts ______________________________________________________________________ ______________________________________________________________________ Title: Methodologies for Evaluating In Situ Bioremediation of ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: U.S. EPA, Research Report, R.S. Kerr Lab. ______________________________________________________________________ ______________________________________________________________________ Date: 1992 ________ NTIS/EPA Document Number(s): EPA/600/R-92/042 _________________________ Page No. 30 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNICAL REFERENCES Author(s): U.S. Environmental Protection Agency ______________________________________________________________________ ______________________________________________________________________ Title: Handbook on In Situ Treatment for Hazardous Waste Contaminant ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: ______________________________________________________________________ ______________________________________________________________________ Date: 1990 ________ NTIS/EPA Document Number(s): _________________________ Page No. 31 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNICAL REFERENCES Author(s): Birge, John ______________________________________________________________________ ______________________________________________________________________ Title: Modern Microbiology Principles And Applications ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: Dubuque, IA ______________________________________________________________________ ______________________________________________________________________ Date: 1992 ________ NTIS/EPA Document Number(s): _________________________ Page No. 32 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B ESTIMATED PRICE RANGE Estimated price range per unit of waste treated : $ 6.00 __________ to $ 8.00 __________ per cubic yard _______________ Price estimates shown above do not always include all indirect costs associated with treatment, such as excavation, permits and treatment of residuals. For price comparisons, users should make certain that vendors provide estimates based on comparable remediation activities. Factors that have a significant effect on unit price. (1 is highest). 3__ Initial contaminant concentration 4__ Moisture content of soil 3__ Target contaminant concentration 2__ Site Preparation 1__ Quantity of waste __ Waste handling/preprocessing 1__ Depth of contamination __ Amount of debris with waste 1__ Depth to ground water 1__ Characteristics of soil 4__ Characteristics of residual waste 3__ Utility/Fuel rates 4__ Labor Rates Others: __________ In-situ treatment is more expensive owing to engineering requirements to move the front of FyreZyme-laden through the soil under toxic conditions. Page No. 33 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TREATABILITY STUDY CAPABILITIES (BENCH-SCALE) Description of bench-scale testing procedures: ---------- Contaminant is divided into two equal amounts, placed in treatment bins, and tested according to protocol. For aerobic treatability testing of soil, the standard FyreZyme protocol is used on the treated sample, and an identical handling without FyreZyme is used on the control. Initial and sequential testing for contaminant levels (TPH, PNA, etc.) is done at 5-day intervals, along with toxicity characteristics leaching (TCLP) or other appropriate tests. Initial bacteriological colony forming units (CFU) counts are done and compared with the sequential test results at 5 to 10 days as indicated. For anaerobic studies on soil or water, similar testing is done in flasks with air replaced by ntirogen or argon gas. These studies will demonstrate the biodegradability of the contaminant. Engineering studies are of course necessary for proper arrangement of wells to move the front of FyreZyme, water, and oxygen through the soil. Can you conduct bench-scale treatability studies on some types of waste at your location: Yes Number of bench-scale studies conducted to date. (Does not include tests on surrogate wastes) : 4_____ Page No. 34 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B PILOT SCALE INFORMATION Pilot unit processes : Pilot-scale studies of treatability are recommended. A standard treatment with FyreZyme is utilized on a representative soil sample, with control samples being run simultaneously. Data are available on similar studies done, comparing FyreZyme with other bioremediation enhancing technologies. Vendor services:_ Equipment manufacture X_ Subcontractor for cleanup services X_ Prime contractor for full-service remediation X_ Other: Consultants for bioremediation Page No. 35 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B PILOT SCALE INFORMATION (Continued) Number of pilot-scale systems : 1_____ Planned/in design 1_____ Under construction 1_____ Constructed Pilot-scale facility is : _ Transportable _ Fixed X_ In situ Capacity range for batch processes: ____________ to ____________ _______________ Can you conduct pilot-scale treatability studies on some types of waste at your location? Yes At a contaminated site? Yes Quantity of waste needed for pilot-scale treatability study : ____________ to ____________ _______________ Number of pilot-scale studies conducted on wastes from different sources or sites : 2_____ Page No. 36 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B FULL SCALE INFORMATION Major unit processes : In Situ Soil Application of FyreZyme A front of FyreZyme is moved through the soil under aerobic conditions, utilizing ground-water gradient-enhancing technology, such as extraction and injection wells, infiltration galleries, sparging, etc. The calcuation for total voulume of FyreZyme to be utilized is the same as that for above ground treatment. Variation in the amount of water to be added as diluent may be necessary, depending on soil saturation, permeability, pH, organic binding capacity, etc. The main costs involved are in the engineering studies to allow for proper placement of wells for injection of the nutrient and any water needed, plus provision of an aerobic environment for maximal bioremediation. Page No. 37 of 37 VISITT 3.0 11/30/99 (s3B Vendor Name: ECOLOGY TECHNOLOGIES INTERNATIONAL, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B FULL SCALE INFORMATION (Continued) Vendor services: _ Equipment manufacture X_ Subcontractor for cleanup services X_ Prime contractor for full-service remediation X_ Other: Consultants for bioremediation Full-scale facility is: _ Transportable _ Fixed X_ In situ Number of full-scale cleanups initiated or completed by this firm using this technology: 2______ Number of full-scale systems: 0_____ Planned/in design 1_____ Under construction 2_____ Constructed Capacity range: ____________ to ____________ _______________ For equipment manufacturers - estimated or actual number of full-scale cleanups by other firms using this equipment: 0______ Page No. 1 of 16 VISITT 3.0 11/30/99 (s3B Vendor Name: MICROBIAL ENVIRONMENTAL SERVICES, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B ________________________________________________________________________ Technology Trade Name: Address: 11280 Aurora Avenue City: Des Moines, Iowa 50322 USA Contact: Jack Sheldon Title: Director, Marketing Phone: (515) 276-3434 Fax: (515) 276-0246 Status: Full scale ________________________________________________________________________ DESCRIPTION OF TECHNOLOGY Microbial Environmental Services, Inc. (MES) has designed several in situ biotreatment systems for treating contaminated soils. The design is based on the introduction of low concentrations of nutritional cofactors and oxygen into the subsurface via infiltration galleries. The treatment is close-loop biodegradation system designed to prevent migration of the contaminants and nutritional cofactors off the treatment site. The results of biodegradation of contaminant by naturally occurring microorganisms are carbon dioxide and water. Monitoring of the vadose zone occurs to watch progress. Page No. 2 of 16 VISITT 3.0 11/30/99 (s3B Vendor Name: MICROBIAL ENVIRONMENTAL SERVICES, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNOLOGY HIGHLIGHTS MES, Inc., performance advantages are the result of site-specific design of biotreatment system and the implementation of intensive quality control and optimized process methodologies. Our on-site bioremediation technologies do not result in the release of volatile organics into the air or allow the leaching of contaminants into the subsurface. Benefits unique to our microbial technologies versus other forms of non-biological remediation include quiet operation, quick and complete degradation of organic contaminants as well as competive price. In addition, our process is completely natural, using only the microorganisms found on site as the degraders. Page No. 3 of 16 VISITT 3.0 11/30/99 (s3B Vendor Name: MICROBIAL ENVIRONMENTAL SERVICES, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNOLOGY LIMITATIONS MES, Inc. bioremediation technologies are not applicable to the treatment of non-biodegradable wastes; wastes containing metals only, inorganic cyanide wastes, inorganic corrosive wastes, and wastes containing radioactive materials. The technologies are also not applicable to the treatment of PCBs and dioxins/furans. Page No. 4 of 16 VISITT 3.0 11/30/99 (s3B Vendor Name: MICROBIAL ENVIRONMENTAL SERVICES, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B OTHER COMMENTS ON TECHNOLOGY Scientists and engineers at MES, Inc. have applied more than a decade of knowledge and experience in bioreactor design, microbial ecology and environmental microbiology, transport phenomena and waste management to develop and design the biotreatment technologies used by MES, Inc. Services offered for cleaning up contaminated soils and groundwater aquifers include: site characterization and biotreatability testing, remedial design, site monitoring and project management. Biotreatment technologies for herbicides/pesticides and explosives are in scale up/demonstration stages. MES, Inc. patents that have been applied for; some patents are still being applied for. Page No. 5 of 16 VISITT 3.0 11/30/99 (s3B Vendor Name: MICROBIAL ENVIRONMENTAL SERVICES, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B WASTE APPLICATIONS Media Actual/Potential X_ X_ Soil (in situ) _ _ Soil (ex situ) _ X_ Sludge (Does not include municipal sewage sludge) _ _ Solid (e.g., slag) _ _ Natural sediment (in situ) _ _ Natural sediment (ex situ) _ _ Ground water (in situ) _ _ Off-gas generated from a primary innovative technology _ _ Dense nonaqueous phase liquids (DNAPL) in situ _ _ Light nonaqueous phase liquids (LNAPL) in situ Page No. 6 of 16 VISITT 3.0 11/30/99 (s3B Vendor Name: MICROBIAL ENVIRONMENTAL SERVICES, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B WASTE APPLICATIONS (Continued) Contaminants and Contaminant Groups Treated Actual/Potential Actual/Potential _ X_ Halogenated volatiles _ _ Heavy metals _ X_ Halogenated semivolatiles _ _ Nonmetallic toxic elements _ X_ Nonhalogenated volatiles _ _ Radioactive metals _ X_ Nonhalogenated semivolatiles _ _ Asbestos _ X_ Organic pesticides/herbicides _ _ Inorganic cyanides _ _ Dioxins/furans _ _ Inorganic corrosives _ _ PCBs _ X_ Polynuclear aromatics (PNAs) Miscellaneous_____________ _ X_ Solvents _ X_ Explosives/propellents X_ X_ Benzene-toluene-ethylbenzene- _ _ Organometallic pesticides/ xylene (BTEX) herbicides _ _ Acetonitrile (organic cyanide) _ _ Organic acids Others: Page No. 7 of 16 VISITT 3.0 11/30/99 (s3B Vendor Name: MICROBIAL ENVIRONMENTAL SERVICES, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B WASTE APPLICATIONS (Continued) Industrial Waste Sources or Site Types Treated Actual/Potential Actual/Potential _ X_ Agriculture _ X_ Munitions manufacturing _ _ Battery recycling/disposal _ X_ Paint/ink formulation _ _ Chloro-alkali manufacturing _ X_ Pesticide manufacturing/use _ X_ Coal gasification X_ X_ Petroleum refining and reuse _ X_ Dry cleaners _ _ Photographic products _ _ Electroplating _ _ Plastics manufacturing X_ X_ Gasoline/service station _ X_ Pulp and paper industry _ X_ Herbicide manufacturing/use _ X_ Other organic chemical manufacturing _ X_ Industrial landfills _ X_ Other inorganic chemical _ _ Inorganic/organic pigments manufacturing _ _ Machine shops _ X_ Rubber manufacturing _ _ Metal ore mining and smelting _ X_ Semiconductor manufacturing _ X_ Municipal landfill _ X_ Wood preserving Others: _ _ Uranium mining ---------- Leaking underground storage tanks Page No. 8 of 16 VISITT 3.0 11/30/99 (s3B Vendor Name: MICROBIAL ENVIRONMENTAL SERVICES, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: City of Carroll ________________________________________ City: Carroll _________________________ State/Province: Iowa ________________ Country: USA _______________ Project Took Place at Site Named: Yes At another Site (i. e., a Test Facility): No Industrial Waste Sources or Site Types Treated _ Agriculture _ Paint/ink formulation _ Battery recycling/disposal _ Pesticide manufacturing/use _ Chloro-alkali manufacturing _ Petroleum refining and reuse _ Coal gasification _ Photographic products _ Dry cleaning _ Plastics manufacturing _ Electroplating _ Pulp and paper industry X_ Gasoline service station _ Other organic chemical manuf. _ Herbicide manufacturing/use _ Other inorganic chemical manufacturing _ Industrial landfills _ Semiconductor manufacturing _ Inorganic/organics pigments _ Rubber manufacturing _ Machine shops _ Wood preserving _ Metal ore mining and smelting _ Uranium mining _ Municipal landfills _ Munitions manufacturing Others: __________ Page No. 9 of 16 VISITT 3.0 11/30/99 (s3B Vendor Name: MICROBIAL ENVIRONMENTAL SERVICES, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: City of Carroll ________________________________________ Regulation/Statute/Organization Media _ RCRA Corrective Action X_ Soil (in situ) _ CERCLA _ Soil (ex situ) _ TSCA _ Sludge _ Safe Drinking Water Act _ Solid X_ UST Corrective Action _ Natural Sediment (in situ) _ DOD _ Natural Sediment (ex situ) _ DOE _ Ground water (in situ) X_ State: Iowa _ Off-gas generated from a primary treatment technology _ Other: _ Dense nonaqueous phase liquid (in situ) _ Not Applicable _ Light nonaqueous phase liquid (in situ) Volume/Quantity Treated: 5,520 __________ cubic yards ____________(units) Area treated (for in situ projects): 23,000 __________ square feet ____________(units) Depth treated (for in sity projects): 10-15 __________ feet ____________(units) Page No. 10 of 16 VISITT 3.0 11/30/99 (s3B Vendor Name: MICROBIAL ENVIRONMENTAL SERVICES, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: City of Carroll ________________________________________ Application Type X_ Full-scale cleanup _ TSCA Research and Development _ Field Demonstration _ EPA SITE Demonstration Program _ Pilot scale treatability study _ EPA SITE Emerging Technology Program _ RCRA Research, Development and Demo _ Research _ TSCA National Demonstration Other: __________ Estimated or actual total and/or unit cost for this project Cost: 20 ____________ Units: cubic yard _________________ Total: NA ____________ Page No. 11 of 16 VISITT 3.0 11/30/99 (s3B Vendor Name: MICROBIAL ENVIRONMENTAL SERVICES, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: City of Carroll ________________________________________ Performance Data Contaminant Untreated Concent. Treated Concent. ----------------------------- --------------------- --------------------- Benzene 1.10 mg/kg <1.0 mg/kg Toluene 11.0 mg/kg <1.0 mg/kg Ethylbenzene 5.5 mg/kg <1.0 mg/kg Xylenes 48.0 mg/kg <1.0 mg/kg TPH 760 mg/kg <5.0 mg/kg Extractable Hydrocarbons (Tot 640.0 mg/kg <5.0 mg/kg Page No. 12 of 16 VISITT 3.0 11/30/99 (s3B Vendor Name: MICROBIAL ENVIRONMENTAL SERVICES, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: City of Carroll ________________________________________ Person outside company familiar with project (optional): Name: ________________________________________ Company: ________________________________________ Address: ________________________________________ ________________________________________ Additional Project Information: _______________ Page No. 13 of 16 VISITT 3.0 11/30/99 (s3B Vendor Name: MICROBIAL ENVIRONMENTAL SERVICES, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B ESTIMATED PRICE RANGE Estimated price range per unit of waste treated : $ 50.00 __________ to $ 125.00 __________ per cubic yard _______________ Price estimates shown above do not always include all indirect costs associated with treatment, such as excavation, permits and treatment of residuals. For price comparisons, users should make certain that vendors provide estimates based on comparable remediation activities. Factors that have a significant effect on unit price. (1 is highest). 3__ Initial contaminant concentration 7__ Moisture content of soil 2__ Target contaminant concentration __ Site Preparation 1__ Quantity of waste 10__ Waste handling/preprocessing 4__ Depth of contamination 8__ Amount of debris with waste 5__ Depth to ground water 9__ Characteristics of soil 6__ Characteristics of residual waste __ Utility/Fuel rates __ Labor Rates Others: __________ Page No. 14 of 16 VISITT 3.0 11/30/99 (s3B Vendor Name: MICROBIAL ENVIRONMENTAL SERVICES, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TREATABILITY STUDY CAPABILITIES (BENCH-SCALE) Description of bench-scale testing procedures: ---------- Several samples of soil and groundwater are characterized for microbial, physical, chemical and geochemical characteristics. Evaluation studies are conducted on the quality and quantity of components in the waste system: mass transfer, material balance on substrate, intermediate and end-products as well as kinetics of biodegradation to establish reaction rates and extent of biodegradation. Specific tests include adsorptivity, transport effects, microbial stimulation studies, microcosms studies, etc. Can you conduct bench-scale treatability studies on some types of waste at your location: Yes Number of bench-scale studies conducted to date. (Does not include tests on surrogate wastes) : 28_____ Page No. 15 of 16 VISITT 3.0 11/30/99 (s3B Vendor Name: MICROBIAL ENVIRONMENTAL SERVICES, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B FULL SCALE INFORMATION Major unit processes : Preprocess: The delivery system that is designed and implemented could include a series of infiltration galleries or soil vapor points. This delivery system is used for the introduction of low concentration nutritional cofactors. Process: The contaminant is broken down by the naturally occurring microorganisms and converted to harmless by-products such as carbon dioxide and water. Post-process: After the appropriate clean-up levels are reached, the system is removed or closed in place. Page No. 16 of 16 VISITT 3.0 11/30/99 (s3B Vendor Name: MICROBIAL ENVIRONMENTAL SERVICES, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B FULL SCALE INFORMATION (Continued) Vendor services: _ Equipment manufacture X_ Subcontractor for cleanup services X_ Prime contractor for full-service remediation _ Other: Full-scale facility is: _ Transportable _ Fixed X_ In situ Number of full-scale cleanups initiated or completed by this firm using this technology: 7______ Number of full-scale systems: 8_____ Planned/in design 1_____ Under construction 6_____ Constructed Capacity range: NA ____________ to ____________ _______________ For equipment manufacturers - estimated or actual number of full-scale cleanups by other firms using this equipment: 0______ Page No. 1 of 14 VISITT 3.0 11/30/99 (s3B Vendor Name: QUATERNARY INVESTIGATIONS, INC. (QI) Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B ________________________________________________________________________ Technology Trade Name: Address: 671 Cooley Drive, Suite 117 City: Colton, California 92324 USA Contact: Tony Morgen Title: President Phone: (909) 423-0740 Fax: (909) 423-0743 Status: Full scale ________________________________________________________________________ DESCRIPTION OF TECHNOLOGY Quaternary Investigations, Inc. (QI) has developed an in-situ bioremediation treatment system for the degradation of chemical compounds in subsurface soils. The system has been used for the degradation of soils contaminated with petroleum hydrocarbons resulting from leaking underground storage tanks. The system allows for treatment of contaminated soils without excavation of the soils and without air emission control requiremenets. Nutrients and microbes are induced into the subsurface soils utilizing a below ground aqueous infiltration unit. Microbe organisms are selected for there capabilities to degradate the specific site contaminants. Page No. 2 of 14 VISITT 3.0 11/30/99 (s3B Vendor Name: QUATERNARY INVESTIGATIONS, INC. (QI) Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNOLOGY HIGHLIGHTS The QI in-situ bioremediation allows for the treatment of a wide variety of compounds in-situ without the excavation of soils. Aromatic, semivolatile and hyrocarbons have been successfully treated using this methodology. The system is economical and can be utilized on small areas such as tank cavities as well as larger contaminated areas. This treatment system has achieved designated clean-up levels in California. In-situ treatment also allows for minimal disturbance of a site while treatment is occuring. Page No. 3 of 14 VISITT 3.0 11/30/99 (s3B Vendor Name: QUATERNARY INVESTIGATIONS, INC. (QI) Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNOLOGY LIMITATIONS The system is limited on the types of compounds (e.g. metals) that are biodegradable and the time period in which they are degradable. The system is more effective in sandy porous soils that allow for nutrient, microbe and oxygen movement. Page No. 4 of 14 VISITT 3.0 11/30/99 (s3B Vendor Name: QUATERNARY INVESTIGATIONS, INC. (QI) Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B OTHER COMMENTS ON TECHNOLOGY The system has been successfully demonstrated at a full-scale level at a site in Southern California. Agencies have approved the design and application of the unit for the treatment of fuel contaminated subsurface soils. No air permitting is required for the unit due to it's in-situ technology and lack of emissions. Page No. 5 of 14 VISITT 3.0 11/30/99 (s3B Vendor Name: QUATERNARY INVESTIGATIONS, INC. (QI) Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B WASTE APPLICATIONS Media Actual/Potential X_ X_ Soil (in situ) _ _ Soil (ex situ) _ _ Sludge (Does not include municipal sewage sludge) _ _ Solid (e.g., slag) _ X_ Natural sediment (in situ) _ _ Natural sediment (ex situ) _ X_ Ground water (in situ) _ _ Off-gas generated from a primary innovative technology _ _ Dense nonaqueous phase liquids (DNAPL) in situ _ _ Light nonaqueous phase liquids (LNAPL) in situ Page No. 6 of 14 VISITT 3.0 11/30/99 (s3B Vendor Name: QUATERNARY INVESTIGATIONS, INC. (QI) Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B WASTE APPLICATIONS (Continued) Contaminants and Contaminant Groups Treated Actual/Potential Actual/Potential _ _ Halogenated volatiles _ _ Heavy metals _ _ Halogenated semivolatiles _ _ Nonmetallic toxic elements _ X_ Nonhalogenated volatiles _ _ Radioactive metals X_ X_ Nonhalogenated semivolatiles _ _ Asbestos _ _ Organic pesticides/herbicides _ _ Inorganic cyanides _ _ Dioxins/furans _ _ Inorganic corrosives _ _ PCBs _ _ Polynuclear aromatics (PNAs) Miscellaneous_____________ _ _ Solvents _ _ Explosives/propellents X_ X_ Benzene-toluene-ethylbenzene- _ _ Organometallic pesticides/ xylene (BTEX) herbicides _ _ Acetonitrile (organic cyanide) _ _ Organic acids Others: Page No. 7 of 14 VISITT 3.0 11/30/99 (s3B Vendor Name: QUATERNARY INVESTIGATIONS, INC. (QI) Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B WASTE APPLICATIONS (Continued) Industrial Waste Sources or Site Types Treated Actual/Potential Actual/Potential _ _ Agriculture _ _ Munitions manufacturing _ _ Battery recycling/disposal _ _ Paint/ink formulation _ _ Chloro-alkali manufacturing _ _ Pesticide manufacturing/use _ X_ Coal gasification _ X_ Petroleum refining and reuse _ _ Dry cleaners _ _ Photographic products _ _ Electroplating _ _ Plastics manufacturing X_ X_ Gasoline/service station _ _ Pulp and paper industry _ _ Herbicide manufacturing/use _ _ Other organic chemical manufacturing _ _ Industrial landfills _ _ Other inorganic chemical _ _ Inorganic/organic pigments manufacturing _ _ Machine shops _ _ Rubber manufacturing _ _ Metal ore mining and smelting _ _ Semiconductor manufacturing _ _ Municipal landfill _ _ Wood preserving Others: _ _ Uranium mining ---------- Page No. 8 of 14 VISITT 3.0 11/30/99 (s3B Vendor Name: QUATERNARY INVESTIGATIONS, INC. (QI) Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Confidential ________________________________________ City: Riverside _________________________ State/Province: California ________________ Country: USA _______________ Project Took Place at Site Named: Yes At another Site (i. e., a Test Facility): No Industrial Waste Sources or Site Types Treated _ Agriculture _ Paint/ink formulation _ Battery recycling/disposal _ Pesticide manufacturing/use _ Chloro-alkali manufacturing _ Petroleum refining and reuse _ Coal gasification _ Photographic products _ Dry cleaning _ Plastics manufacturing _ Electroplating _ Pulp and paper industry X_ Gasoline service station _ Other organic chemical manuf. _ Herbicide manufacturing/use _ Other inorganic chemical manufacturing _ Industrial landfills _ Semiconductor manufacturing _ Inorganic/organics pigments _ Rubber manufacturing _ Machine shops _ Wood preserving _ Metal ore mining and smelting _ Uranium mining _ Municipal landfills _ Munitions manufacturing Others: __________ Page No. 9 of 14 VISITT 3.0 11/30/99 (s3B Vendor Name: QUATERNARY INVESTIGATIONS, INC. (QI) Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Confidential ________________________________________ Regulation/Statute/Organization Media _ RCRA Corrective Action X_ Soil (in situ) _ CERCLA _ Soil (ex situ) _ TSCA _ Sludge _ Safe Drinking Water Act _ Solid X_ UST Corrective Action _ Natural Sediment (in situ) _ DOD _ Natural Sediment (ex situ) _ DOE _ Ground water (in situ) X_ State: California _ Off-gas generated from a primary treatment technology _ Other: _ Dense nonaqueous phase liquid (in situ) _ Not Applicable _ Light nonaqueous phase liquid (in situ) Volume/Quantity Treated: __________ ____________(units) Area treated (for in situ projects): 1,000 __________ square feet ____________(units) Depth treated (for in sity projects): 12 __________ feet ____________(units) Page No. 10 of 14 VISITT 3.0 11/30/99 (s3B Vendor Name: QUATERNARY INVESTIGATIONS, INC. (QI) Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Confidential ________________________________________ Application Type X_ Full-scale cleanup _ TSCA Research and Development X_ Field Demonstration _ EPA SITE Demonstration Program _ Pilot scale treatability study _ EPA SITE Emerging Technology Program _ RCRA Research, Development and Demo _ Research _ TSCA National Demonstration Other: __________ Estimated or actual total and/or unit cost for this project Cost: ____________ Units: _________________ Total: 25,000 ____________ Page No. 11 of 14 VISITT 3.0 11/30/99 (s3B Vendor Name: QUATERNARY INVESTIGATIONS, INC. (QI) Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Confidential ________________________________________ Performance Data Contaminant Untreated Concent. Treated Concent. ----------------------------- --------------------- --------------------- Volatile Petroleum Hydrocarbo 4500 ppm ppm Page No. 12 of 14 VISITT 3.0 11/30/99 (s3B Vendor Name: QUATERNARY INVESTIGATIONS, INC. (QI) Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Confidential ________________________________________ Person outside company familiar with project (optional): Name: Confidential ________________________________________ Company: Confidential ________________________________________ Address: ________________________________________ ________________________________________ Additional Project Information: _______________ Page No. 13 of 14 VISITT 3.0 11/30/99 (s3B Vendor Name: QUATERNARY INVESTIGATIONS, INC. (QI) Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B FULL SCALE INFORMATION Major unit processes : o Infiltration gallery o Mixing/storage tanks for nutrient and microbe addition o Misc. pumps and piping Enhanced bioremediation is conducted through the addition of nutrients and microbes into the soils. These are added in an aqueous media through a subsurface infiltration gallery. Oxygen levels can be enhanced chemically or using QI's biooxidation system. Treatment efficiency is monitored by carbon dioxide and oxygen levels, microbe levels, nutrient and contaminant levels analyzed from the aqueous media obtained from the soil through piezometers. Page No. 14 of 14 VISITT 3.0 11/30/99 (s3B Vendor Name: QUATERNARY INVESTIGATIONS, INC. (QI) Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B FULL SCALE INFORMATION (Continued) Vendor services: _ Equipment manufacture X_ Subcontractor for cleanup services X_ Prime contractor for full-service remediation _ Other: Full-scale facility is: _ Transportable _ Fixed X_ In situ Number of full-scale cleanups initiated or completed by this firm using this technology: 1______ Number of full-scale systems: 1_____ Planned/in design 0_____ Under construction 1_____ Constructed Capacity range: ____________ to ____________ _______________ For equipment manufacturers - estimated or actual number of full-scale cleanups by other firms using this equipment: 0______ Page No. 1 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B ________________________________________________________________________ Technology Trade Name: DUAL AUGER SYSTEM Address: PO Box 516 City: Chandler, Arizona 85244-0516 USA Contact: Mr. Richard P. Murray Title: President Phone: (602) 821-0409 Fax: (602) 786-3184 Status: Full scale ________________________________________________________________________ DESCRIPTION OF TECHNOLOGY This process increases the quality and acceleration of biodegradation in deep contaminated soils. The specialized dual auger equipment system injects site specific microorganism mixtures, along with the required nutrients and homogeneously mixes them into the contaminated soils, without requiring any excavation. The injection and mixing process effectively breaks down fluid and soil strata barriers and eliminates pockets of contaminated soil that would otherwise remain untreated, thus assuring a 100 percent treated area. Oxygen, water, nutrients, and/or natural bacteria, if required, are injected into the contaminated area, as determined by a site specific laboratory test program. The process uses a twin, 5-foot diameter auger system powered and moved by a standard backhoe. The hollow-shaft auger drills into the contaminated soil, allowing the microorganism and nutrient mixtures to be continually injected through a controlled nozzle system. The distribution of the microorganisms and nutrients occurs during the initial auger action. The auger flights break the soil loose, allowing mixing blades to thoroughly blend the microorganism and nutrient mixture with the soil. This occurs in an overlapping manner, to ensure the complete treatment of all contaminated soil. The mixing action is continued as the augers are withdrawn. Treatment depth can exceed 100 feet. Upon completion of the injection and mixing process, a simple oxygen supplement system is installed. RESIDUALS/WASTES: There are no residuals or wastes generated in our process, since all the treatment is performed beneath the ground surface. Upon completion of the remediation operations, the treated area can be returned to its original service. Page No. 2 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNOLOGY HIGHLIGHTS The Dual Auger System can be used for any application that requires delivery of material below the surface. For example, the Dual Auger System can be used for constructing barrier walls or for in situ delivery of chemicals for chemical treatment or stabilization. Page No. 3 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNOLOGY LIMITATIONS Since our process is dependent upon the injection and mixing of the reagents, through the use of dual auger equipment, large buried objects (car bodies, tree stumps, large concrete slabs, and boulders over 18") will limit the processes' capabilities of treating 100 percent of a contaminated area. Page No. 4 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B OTHER COMMENTS ON TECHNOLOGY In a continuing effort to reduce the time required for biodegradation, In-Situ Fixation, Inc. is currently engaged in a pilot program, which includes the incorporation of "time release" oxygen agent into our injection process. The incorporation of prolonged oxygen release agents will help accelerate biodegradation and reduce the overall remediation time period. Page No. 5 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B WASTE APPLICATIONS Media Actual/Potential X_ X_ Soil (in situ) _ _ Soil (ex situ) X_ X_ Sludge (Does not include municipal sewage sludge) _ _ Solid (e.g., slag) _ X_ Natural sediment (in situ) _ _ Natural sediment (ex situ) _ X_ Ground water (in situ) _ _ Off-gas generated from a primary innovative technology _ X_ Dense nonaqueous phase liquids (DNAPL) in situ _ X_ Light nonaqueous phase liquids (LNAPL) in situ Page No. 6 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B WASTE APPLICATIONS (Continued) Contaminants and Contaminant Groups Treated Actual/Potential Actual/Potential X_ X_ Halogenated volatiles _ _ Heavy metals X_ X_ Halogenated semivolatiles _ _ Nonmetallic toxic elements X_ X_ Nonhalogenated volatiles _ _ Radioactive metals X_ X_ Nonhalogenated semivolatiles _ _ Asbestos X_ X_ Organic pesticides/herbicides _ _ Inorganic cyanides _ X_ Dioxins/furans _ _ Inorganic corrosives _ X_ PCBs _ X_ Polynuclear aromatics (PNAs) Miscellaneous_____________ X_ X_ Solvents _ _ Explosives/propellents X_ X_ Benzene-toluene-ethylbenzene- _ X_ Organometallic pesticides/ xylene (BTEX) herbicides _ _ Acetonitrile (organic cyanide) _ _ Organic acids Others: Page No. 7 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B WASTE APPLICATIONS (Continued) Industrial Waste Sources or Site Types Treated Actual/Potential Actual/Potential _ X_ Agriculture _ _ Munitions manufacturing _ _ Battery recycling/disposal _ X_ Paint/ink formulation _ _ Chloro-alkali manufacturing _ X_ Pesticide manufacturing/use _ _ Coal gasification _ X_ Petroleum refining and reuse _ X_ Dry cleaners _ X_ Photographic products _ _ Electroplating _ X_ Plastics manufacturing _ _ Gasoline/service station _ X_ Pulp and paper industry _ X_ Herbicide manufacturing/use _ _ Other organic chemical manufacturing _ X_ Industrial landfills X_ X_ Other inorganic chemical _ X_ Inorganic/organic pigments manufacturing _ X_ Machine shops _ X_ Rubber manufacturing _ _ Metal ore mining and smelting _ X_ Semiconductor manufacturing _ X_ Municipal landfill _ X_ Wood preserving Others: _ _ Uranium mining ---------- Page No. 8 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Automobile Dealership ________________________________________ City: Yuma _________________________ State/Province: Arizona ________________ Country: USA _______________ Project Took Place at Site Named: Yes At another Site (i. e., a Test Facility): No Industrial Waste Sources or Site Types Treated _ Agriculture _ Paint/ink formulation _ Battery recycling/disposal _ Pesticide manufacturing/use _ Chloro-alkali manufacturing _ Petroleum refining and reuse _ Coal gasification _ Photographic products _ Dry cleaning _ Plastics manufacturing _ Electroplating _ Pulp and paper industry X_ Gasoline service station _ Other organic chemical manuf. _ Herbicide manufacturing/use _ Other inorganic chemical manufacturing _ Industrial landfills _ Semiconductor manufacturing _ Inorganic/organics pigments _ Rubber manufacturing _ Machine shops _ Wood preserving _ Metal ore mining and smelting _ Uranium mining _ Municipal landfills _ Munitions manufacturing Others: __________ Page No. 9 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Automobile Dealership ________________________________________ Regulation/Statute/Organization Media _ RCRA Corrective Action X_ Soil (in situ) _ CERCLA _ Soil (ex situ) _ TSCA _ Sludge _ Safe Drinking Water Act _ Solid _ UST Corrective Action _ Natural Sediment (in situ) _ DOD _ Natural Sediment (ex situ) _ DOE _ Ground water (in situ) X_ State: Arizona _ Off-gas generated from a primary treatment technology X_ Other: ADEQ _ Dense nonaqueous phase liquid (in situ) _ Not Applicable _ Light nonaqueous phase liquid (in situ) Volume/Quantity Treated: 6,000 __________ cubic yards ____________(units) Area treated (for in situ projects): 6,000 __________ square feet ____________(units) Depth treated (for in sity projects): 6,000 __________ feet ____________(units) Page No. 10 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Automobile Dealership ________________________________________ Application Type X_ Full-scale cleanup _ TSCA Research and Development _ Field Demonstration _ EPA SITE Demonstration Program _ Pilot scale treatability study _ EPA SITE Emerging Technology Program _ RCRA Research, Development and Demo _ Research _ TSCA National Demonstration Other: __________ Estimated or actual total and/or unit cost for this project Cost: 17 - 23 ____________ Units: cubic yard _________________ Total: 120,000.00 ____________ Page No. 11 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Automobile Dealership ________________________________________ Performance Data Contaminant Untreated Concent. Treated Concent. ----------------------------- --------------------- --------------------- TPH 500 -40,000 ppm current-in prog ppm Page No. 12 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B REPRESENTATIVE PROJECTS Site Name or Industry Type if Client Identity Confidential: Automobile Dealership ________________________________________ Person outside company familiar with project (optional): Name: ________________________________________ Company: ________________________________________ Address: ________________________________________ ________________________________________ Additional Project Information: _______________ Soil conditions - sandy soil with a 12 foot clay layer at approximately 30 to 42 feet. Confined area near adjacent structures, excavation would require extensive shoring, requiring tie-backs. Normal business operations were able to continue during remediation operations. Page No. 13 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TECHNICAL REFERENCES Author(s): In-Situ Fixation, Inc. ______________________________________________________________________ ______________________________________________________________________ Title: Deep Soil In-Situ Bioremediation ________________________________________________________________________ ________________________________________________________________________ Journal/Conference: Report & Video Tape ______________________________________________________________________ ______________________________________________________________________ Date: 1993 ________ NTIS/EPA Document Number(s): _________________________ Page No. 14 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B ESTIMATED PRICE RANGE Estimated price range per unit of waste treated : $ 15.00 __________ to $ 75.00 __________ per cubic yard _______________ Price estimates shown above do not always include all indirect costs associated with treatment, such as excavation, permits and treatment of residuals. For price comparisons, users should make certain that vendors provide estimates based on comparable remediation activities. Factors that have a significant effect on unit price. (1 is highest). 3__ Initial contaminant concentration 4__ Moisture content of soil 2__ Target contaminant concentration __ Site Preparation __ Quantity of waste 1__ Waste handling/preprocessing 6__ Depth of contamination 7__ Amount of debris with waste __ Depth to ground water 5__ Characteristics of soil __ Characteristics of residual waste __ Utility/Fuel rates __ Labor Rates Others: __________ Page No. 15 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B TREATABILITY STUDY CAPABILITIES (BENCH-SCALE) Description of bench-scale testing procedures: ---------- BENCH-SCALE TEST PROCEDURE 1. The characterization and concentration of the contaminant(s) must be identified and determined to be biodegradable. (This can be performed by the owner or owners representative). 2. The existing soil conditions are examined to ensure that the contaminated environment has the ability to sustain and achieve successful bioremediation. 3. Confirm that a sufficient population of naturally occurring bacteria are present or whether additional natural bacteria must be included in the injection process. 4. (Optional) Create a laboratory environment simulating the existing site conditions, perform laboratory tests, utilizing our specially designed 1/10 scale laboratory model of our dual auger and soil columns, to simulate actual field operations. Once the soil columns have been treated, a monitoring and sample program is commenced to record the biodegradation rate. Can you conduct bench-scale treatability studies on some types of waste at your location: Yes Number of bench-scale studies conducted to date. (Does not include tests on surrogate wastes) : 5_____ Page No. 16 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B PILOT SCALE INFORMATION Pilot unit processes : After the site-specific bench-scale reagent design has been performed and the site specific reagent(s) determined, a pilot-scale test is performed. The pilot-scale test utilizes the full-scale remediation equipment system and is performed on site, under actual field conditions. The results of the pilot-scale test program are measured against the bench-scale results and the project clean-up levels, to ensure the success of the full-scale remediation. Vendor services:X_ Equipment manufacture X_ Subcontractor for cleanup services X_ Prime contractor for full-service remediation _ Other: Page No. 17 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B PILOT SCALE INFORMATION (Continued) Number of pilot-scale systems : 0_____ Planned/in design 0_____ Under construction 1_____ Constructed Pilot-scale facility is : _ Transportable _ Fixed X_ In situ Capacity range for batch processes: ____________ to ____________ _______________ Can you conduct pilot-scale treatability studies on some types of waste at your location? No At a contaminated site? Yes Quantity of waste needed for pilot-scale treatability study : 100 ____________ to 300 ____________ cubic yards _______________ Number of pilot-scale studies conducted on wastes from different sources or sites : 1_____ Page No. 18 of 19 VISITT 3.0 11/30/99 (s3B Vendor Name: IN-SITU FIXATION, INC. Technology Type: BIOREMEDIATION - IN SITU SOIL (s0B FULL SCALE INFORMATION Major unit processes : Prior to full-scale operations, bench-scale testing of the actual site contaminated soils is performed, utilizing our 1/10 scale laboratory model of the dual auger system. This pre-operational reagent design and testing phase helps insure that the full-scale operational phase can meet the project clean-up goals. After the laboratory bench-scale testing has determined the site specific injection reagents, a pilot-scale test is run to determine the optimum injection rate. Reagents and water are mixed in a mixing tank and then transferred to the reservoir tank. From the reservoir tank, the liquid reagents are metered and pumped to the dual auger equipment. The dual auger equipment injects the liquid reagent into the contaminated soil at a rate previously determined during the pilot test program. During the full scale remediation operation, a comprehensive quality control/quality assurance program is followed to ensure complete treatment of the contaminated area. The program involves continuous reagent monitoring and field sampling to ensure optimum conditions are maintained during the full-scale operation. Also see the general description of the technology.